SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Identificación molecular de heces y análisis de hábitos alimenticios de carnívoros en la reserva de la biósfera "Sierra del Abra Tanchipa", San Luis Potosí, México.

Dolores Ramos Eva.

Con el objeto de estandarizar y perfeccionar una técnica de extracción y purificación de ADN a partir de sangre, semen y pelo de porcinos, se obtuvieron muestras de 35 sementales de línea materna Yorkshire y Landrace, en los cuales se valoró la frecuencia de los genes ESR y PRLR mediante PCR-RFLP. La extracción de ADN a partir de sangre y semen únicamente se estandarizaron. El pelo de sementales fue la mejor opción para obtener una alta cantidad y calidad de ADN. Se identificó la frecuencia de dos genes relacionados con prolificidad: Receptor de Prolactina (PRLR) y Receptor de Estrógeno (ESR); en su amplificación se utilizó PCR-RFLP´s y se identificaron los genotipos. En esta identificación se utilizó la enzima Alu I para PRLR y Pvu II para...

Palavras-chave: ADN; Pelo; Sangre; Semen; Cerdos; PCR-RFLP hair; Blood and semen DNA; Boars; PCR-RFLP.

Ano: 2012

URL: http://hdl.handle.net/10521/911

Benítez Alemán, Héctor Eduardo.

La identificación precisa de heces de carnívoros silvestres es importante para describir sus hábitos alimenticios, sobre todo en aquellas especies elusivas o en riesgo. Sin embargo Identificar las heces de especies de carnívoros simpátricos no es una tarea sencilla. Por ello, las técnicas moleculares, son herramientas que brindan mayor precisión en la identificación de heces. El análisis de hábitos alimenticios aporta información valiosa para entender la estructura poblacional de los depredadores y sus presas, así como su importancia ecológica para generar estrategias de manejo y conservación de carnívoros y sus presas. Los objetivos de este estudio fueron identificar las heces de carnívoros extrayendo ADN mitocondrial de estas y amplificando un fragmento...

Palavras-chave: ADN mitocrondrial; Citocromo b; PCR-RFLP; Hábitos alimenticios; Carnívoros; Mitochondrial DNA; Cytochrome b; Food habits; Carnivores; Ganadería; Maestría.

Ano: 2014

URL: http://hdl.handle.net/10521/2291

Estandarización de técnicas de extracción de ADN en sementales porcinos para evaluar la frecuencia de los genes ESR y PLRL con PCR-RFLP

Estandarización de técnicas de extracción de ADN en sementales porcinos para evaluar la frecuencia de los genes ESR y PLRL con PCR-RFLP

Dolores Ramos Eva.

Tipo: Tesis

Palavras-chave: ADN; Pelo; Sangre; Semen; Cerdos; PCR-RFLP; Hair; Blood and semen DNA; Boars.

Ano: 2007

URL: http://hdl.handle.net/10521/1202

Genetic polymorphism of the αs1-casein locus in five populations of goats from Mexico

Dolores Ramos Eva.

Palavras-chave: ADN; Pelo; Sangre; Semen; Cerdos; PCR-RFLP hair; Blood and semen DNA; Boars; PCR-RFLP.

Ano: 2012

URL: http://hdl.handle.net/10521/888

Torres-Vázquez,José Antonio; Vázquez Flores,Felícitas; Montaldo,Hugo H; Ulloa-Arvizu,Raúl; Valencia Posadas,Mauricio; Gayosso Vázquez,Amanda; Alonso Morales,Rogelio Alejandro.

With the objective of estimating allele frequencies, and testing for population divergence for the CSN1S1 locus, genotypes of animals from five goat populations; Saanen (n = 97), Alpine (n = 81) Toggenburg (n = 92), local goats with external appearance similar to the Murciana-Granadina breed from Central Mexico (n = 26) and heterogeneous local animals denominated Mosaico Lagunero (n = 30), from Northern Mexico, were identified using PCR and Xmn1 PCR-RFLP methodology. For Saanen, Alpine and Toggenburg, the sum of E and F alleles had the largest frequencies (from 0.468 to 0.789), while for the groups local Murciana-Granadina and Mosaico Lagunero the sum of the most frequent allelic groups (A* and B*), were 0.385 and 0.533 respectively. Both local...

Tipo: Journal article

Palavras-chave: CSN1S1; Gene frequencies; Genetic diversity; Goat milk; PCR-RFLP.

Ano: 2008

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300007

A C1069G SNP of the MC4R gene and its association with economic traits in Korean native cattle (brown, brindle, and black)

Polymorphisms of the bovine chemokine receptor-like 1 gene and their associations with meat quality traits in Qinchuan cattle (Bos taurus)

Lee,Youngsub; Park,Sairom; Kim,Hun; Lee,Seung Kyu; Kim,Jin Woo; Lee,Hak Kyo; Jeong,Dong Kee; Lee,Sung Jin.

Background: The melanocortin-4 receptor gene (MC4R) plays an important role in regulating food intake and body weight in mammals. In the present study, we identified the MC4R gene in native Korean brown, brindle, and black cattle by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP), and investigated its association with economic traits. A total of 413 cattle from the three breeds were tested for backfat thickness, carcass weight, longissimus muscle area, and marbling score, and statistical data were analyzed using the SAS program. Results: The C allele had the highest frequency in brown and brindle cattle, and the G allele frequency was highest in black cattle. The C1069G SNP was significantly (p < 0.05) associated with only...

Tipo: Journal article

Palavras-chave: Economic trait; Marbling scores; MC4R gene; PCR-RFLP; SNP.

Ano: 2013

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500014

Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle

Zhan,Xiaoli; Jiang,Bijie; Ma,Xianghui; Fu,Changzhen; Zan,Linsen.

Background: We investigated the polymorphisms of the bovine chemokine receptor-like 1(CMKLR1) gene. The coding region of CMKLR1 was screened in Qinchuan cattle by PCR-RFLP technology. Results: In this study, we discovered two single nucleotide polymorphisms (SNPs) (264G > C and 762C > T) in the coding region of the CMKLR1 gene. Hence, we described the BmgT120l and Pdm1 PCR-RFLP methods for detecting the 64G > C and 762C > T mutations, respectively. PCR-RFLP and sequencing were used to analyze the two loci of CMKLR1 gene in 324 individuals, which were randomly selected from breeding populations. Furthermore, meat quality traits in another 80 Qinchuan individuals were analyzed by the comparison between the genotypes and their phenotypic data....

Tipo: Journal article

Palavras-chave: CMKLR1 gene; Meat quality traits; PCR-RFLP; Qinchuan cattle; SNP.

Ano: 2013

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000100003

Polymorphism of locus DRB3.2 in populations of Creole Cattle from Northern Mexico

Fu,Changzhen; Wang,Hong; Li,Yaokun; Wang,Jiali; Cheng,Gong; Wang,Hongbao; Yang,Wucai; Zan,Linsen.

Background CDIPT (CDP-diacylglycerol-inositol 3-phosphatidyltransferase, EC 2.7.8.11) was found on the cytoplasmic side of the Golgi apparatus and the endoplasmic reticulum. It was an integral membrane protein performing the last step in the de novo biosynthesis of phosphatidylinositol (PtdIns). In recent years, PtdIns has been considered to play an essential role in energy metabolism, fatty acid metabolic pathway and intracellular signal transduction in eukaryotic cells. Results In this study, the results of real-time polymerase chain reaction (PCR) showed that the expression of CDIPT gene was remarkably different in diverse tissues. We also detected the polymorphism of bovine CDIPT gene and analyzed its association with body measurement and meat quality...

Tipo: Journal article

Palavras-chave: Body measurement traits; Gene expression; Meat quality traits; PCR-RFLP; SNP.

Ano: 2014

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400004

Fernández,Ilda G.; Ramírez,José Gonzalo Ríos; Vázquez,Amanda Gayosso; Arvizu,Raúl Ulloa; Morales,Rogelio A. Alonso.

The polymorphism of locus BoLA-DRB3.2 of the Major Histocompatibility Complex was evaluated in two northern Mexican Creole cattle populations, Chihuahua (n = 47) and Tamaulipas (n = 51). The BoLA-DRB3.2 locus was typed by amplification and digestion with restriction endonuclease enzymes (PCR-RFLP). Fifty-two alleles were detected (28 previously reported and 24 new ones). In the Chihuahua population, 18 alleles and 5.5 effective alleles were found, while in the Tamaulipas population there were 34 and 10.8, respectively. The allele frequencies ranged from 0.011 to 0.383 in Chihuahua and from 0.010 to 0.206 in Tamaulipas. The frequencies of the new alleles in both cattle populations were low (0.010 to 0.053). The expected heterozygosity was 0.827 and 0.916,...

Palavras-chave: BoLA-DRB3.2; Polymorphism; Creole cattle; PCR-RFLP.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500013

High levels of genetic variability and differentiation in hilsa shad, Tenualosa ilisha (Clupeidae, Clupeiformes) populations revealed by PCR-RFLP analysis of the mitochondrial DNA D-loop region

Mazumder,Sabuj Kanti; Alam,Md. Samsul.

The hilsa shad, Tenualosa ilisha (Clupeidae, Clupeiformes) is an important anadromous clupeid species from the Western division of the Indo-Pacific region. It constitutes the largest single fishable species in Bangladesh. Information on genetic variability and population structure is very important for both management and conservation purposes. Past reports on the population structure of T. ilisha involving morphometric, allozyme and RAPD analyses are contradictory. We examined genetic variability and divergence in two riverine (the Jamuna and the Meghna), two estuarine (Kuakata and Sundarbans) and one marine (Cox's Bazar) populations of T. ilisha by applying PCR-RFLP analysis of the mtDNA D-loop region. The amplified PCR products were restricted with four...

Aromatase gene and its effects on growth, reproductive and maternal ability traits in a multibreed sheep population from Brazil

Palavras-chave: Genetic variability; PCR-RFLP; MtDNA; Tenualosa ilisha.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100029

Lôbo,Ana Maria Bezerra Oliveira; Lôbo,Raimundo Nonato Braga; Paiva,Samuel Rezende.

We determined the polymorphism C242T of the aromatase gene (Cyp19) and its allelic frequency, as well as the effect of the variants on productive and reproductive traits in 71 purebred Santa Inês sheep, 13 purebred Brazilian Somali sheep, nine purebred Poll Dorset sheep, and 18 crossbred 1/2 Dorper sheep. The animals were genotyped using the PCR-RFLP technique. The influence of the animal's genotype on its performance or on the performance of its lambs was analyzed by the least square method. Another factor assessed was the importance of the animal's genotype in analysis models for quantitative breeding value estimates, and whether there were differences among the averages of breeding values of animals with different genotypes for this gene. In the sample...

High frequency of the HRAS oncogene codon 12 mutation in Macedonian patients with urinary bladder cancer

Palavras-chave: Body weight; Lambing interval; PCR-RFLP; Polymorphism; SNP; Litter weight.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300010

Panov,Sasho; Roganovic-Zafirova,Danica; Stavric,George; Yashar,Genghis; Popov,Zivko.

Point mutations at codon 12 of the HRAS (v-Ha-ras Harvey rat sarcoma viral oncogene homolog) oncogene are one of the best defined and widely studied molecular genetic events in transitional cell carcinoma (TCC) of the urinary bladder. The aim of this study was to use the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of paraffin-embedded tissue-derived DNA to determine the frequency of the HRAS oncogene G ->T codon 12 mutation in TCC patients being treated at the University Urology Clinic in Skopje, Republic of Macedonia. DNA isolated from paraffin-embedded tissue (PET) surgically removed TCC specimens of 62 (81.58%) out of 76 patients were successfully amplified, the remaining 14 (18.42%) showing compromised DNA...

A modified simple RFLP-PCR method for single nucleotide polymorphism (SNP) typing

Palavras-chave: HRAS; Oncogene; Bladder cancer; PCR-RFLP.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100002

Xiao,Junhua; Xin,Xiujuan; Luan,Xiaohui; Dongzhi,Wei; Shengli,Yang.

We describe a modified single nucleotide polymorphism (SNP) typing method based on the restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR). This is a simple, economical method without the need for special equipment. For most SNP loci, a common restriction endonuclease (Hind III, EcoR I or BamH I) recognizing site (RER) can be introduced into one allelic form, but not the other by two rounds of mismatched PCR. The flanking regions can be changed by as many as five bases after PCR amplification with specially designed mismatching primers so the genotypes can be distinguished after digestion of the PCR products with corresponding endonucleases.

The use of PCR-RFLP as an identification tool for three closely related species of rodents of the genus Akodon (Sigmodontinae, Akodontini)

Palavras-chave: SNP genotyping; PCR-RFLP; RER site.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300028

Fagundes,Valéria; Nogueira,Cristina Dornelas de Andrade.

Three cryptic species of the rodent Akodon (A. cursor, A. montensis and Akodon sp.) were analyzed. The two former species are sympatric in the Brazilian states of São Paulo, Rio de Janeiro and Minas Gerais, where hybrids have already been found. The third species, Akodon sp., occurs in an isolated area in Central Brazil. The identification of these species is difficult by the need of living animals. At present, karyotyping is the only method used in the identification of specimens. We used PCR-RFLP of the mitochondrial cytochrome gene to test the distinctiveness of the three species, which was confirmed by the absence of shared species-specific haplotypes. We also detected a geographical pattern of haplotypes distribution with highly polymorphic...

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Akodon cursor; Akodon montensis; PCR-RFLP; Sigmodontinae.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400031

Association of the bovine major histocompatibility complex (BoLA) BoLA-DRB3 gene with fat and protein production and somatic cell score in Brazilian Gyr dairy cattle (Bos indicus)

Nascimento,Carlos Souza do; Machado,Marco Antonio; Martinez,Mario Luiz; Silva,Marcos Vinícius G. Barbosa da; Guimarães,Marta Fonseca Martins; Campos,Ana Lúcia; Azevedo,Ana Luisa Sousa; Teodoro,Roberto Luiz; Verneque,Rui da Silva; Guimarães,Simone Eliza Facioni; Oliveira,Denise Aparecida Andrade.

The effect of the bovine major histocompatibility complex (BoLA) locus on animal health may be due to a direct action of its alleles on immune functions, whereas its indirect effect on production traits might be explained by the better general health conditions of more productive animals. In the present study, the BoLA-DRB3 gene was investigated in 1058 cows belonging to seven Brazilian Gyr Dairy herds (Bos indicus, Zebu cattle). A total of 37 alleles were identified, 15 of them described for the first time in a Zebu breed. A highly significant association (p < 0.02) was observed between allele *54 and a decrease (-26.1 kg) in milk protein yield and there was a significant association (p < 0.05) between this allele and lower (-26.07 kg) milk fat...

Superoxide dismutase, catalase, glutathione peroxidase and gluthatione S-transferases M1 and T1 gene polymorphisms in three Brazilian population groups

Palavras-chave: Cattle; Marker-assisted selection; Molecular markers; PCR-RFLP.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400011

Hiragi,Cássia de Oliveira; Miranda-Vilela,Ana Luisa; Rocha,Dulce Maria Sucena; ,Silviene Fabiana de; Hatagima,Ana; Klautau-Guimarães,Maria de Nazaré.

Antioxidants such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX1) reduce the oxidation rates in the organism. Gluthatione S-transferases (GSTs) play a vital role in phase 2 of biotransformation of many substances. Variation in the expression of these enzymes suggests individual differences for the degree of antioxidant protection and geographical differences in the distribution of these variants. We described the distribution frequency of CAT (21A/T), SOD2 (Ala9Val), GPX1 (Pro198Leu), GSTM1 and GSTT1 polymorphisms in three Brazilian population groups: Kayabi Amerindians (n = 60), Kalunga Afro-descendants (n = 72), and an urban mixed population from Federal District (n = 162). Frequencies of the variants observed in Kalunga...

Detection of Metarhizium anisopliae var. anisopliae within infected sugarcane borer Diatraea saccharalis (Lepidoptera, Pyralidae) using specific primers

Palavras-chave: Antioxidants; PCR-RFLP; Gene polymorphisms; Brazilian ethnicities; Population genetics; Pharmacogenetics.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100003

Destéfano,Ricardo Henri Rodrigues; Destéfano,Suzete A. Lanza; Messias,Claudio Luiz.

In order to construct specific primers for the detection and identification of the entomopathogenic fungus Metarhizium within infected sugarcane borer (Diatraea saccharalis) larvae we analyzed the ITS1 -5.8S- ITS2 rDNA regions of strains and varieties of M. anisopliae, M. album and M. flavoviride. The PCR amplification of these regions yielded a unique fragment of approximately 540 bp for M. anisopliae variety anisopliae strains E9, B/Vi and C (isolated in Brazil), 600 pb for M. a. anisopliae strain 14 (isolated in Australia), 650 bp for the M. album and 600 bp for M. flavoviride strains. The PCR products were digested with different restriction endonucleases (Afa I, Alu I, Dde I, Hae III, Hpa II and Sau 3A) and the PCR-RFLP profiles showed clear...

Reconstruction of major maternal and paternal lineages of the Cape Muslim population

Palavras-chave: Metarhizium anisopliae; Entomopathogenic fungi; PCR-RFLP; ITS region.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200020

Isaacs,Shafieka; Geduld-Ullah,Tasneem; Benjeddou,Mongi.

The earliest Cape Muslims were brought to the Cape (Cape Town -South Africa) from Africa and Asia from 1652 to 1834. They were part of an involuntary migration of slaves, political prisoners and convicts, and they contributed to the ethnic diversity of the present Cape Muslim population of South Africa. The history of the Cape Muslims has been well documented and researched however no in-depth genetic studies have been undertaken. The aim of the present study was to determine the respective African, Asian and European contributions to the mtDNA (maternal) and Y-chromosomal (paternal) gene pool of the Cape Muslim population, by analyzing DNA samples of 100 unrelated Muslim males born in the Cape Metropolitan area. A panel of six mtDNA and eight Y-chromosome...

Polymorphism in the internal transcribed spacer (ITS) of the ribosomal DNA of 26 isolates of ectomycorrhizal fungi

Palavras-chave: PCR-RFLP; Genetic polymorphism; Mitochondrial DNA; Population genetic structure; Chromosome variations.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200005

Gomes,Eliane A.; Kasuya,Maria Catarina M.; Barros,Everaldo G. de; Borges,Arnaldo C.; Araújo,Elza F..

Inter- and intraspecific variation among 26 isolates of ectomycorrhizal fungi belonging to 8 genera and 19 species were evaluated by analysis of the internal transcribed sequence (ITS) of the rDNA region using restriction fragment length polymorphism (RFLP). The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and then cleaved with different restriction enzymes. Amplification products, which ranged between 560 and 750 base pairs (bp), were obtained for all the isolates analyzed. The degree of polymorphism observed did not allow proper identification of most of the isolates. Cleavage of amplified fragments with the restriction enzymes Alu I, Hae III, Hinf I, and Hpa II revealed extensive polymorphism. All eight genera...

Association of polymorphisms in the heparanase gene (HPSE) with hepatocellular carcinoma in Chinese populations

Palavras-chave: DNA fingerprinting; Ectomycorrhizal fungi; Internal transcribed spacer (ITS); PCR-RFLP; Ribosomal DNA (rDNA).

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400018

Yu,Lixia; Zhang,Xiaoai; Zhai,Yun; Zhang,Hongxing; Yue,Wei; Zhang,Xiumei; Wang,Zhifu; Zhou,Hong; Zhou,Gangqiao; Gong,Feng.

Abstract Heparanase activity is involved in cancer growth and development in humans and single nucleotide polymorphisms (SNPs) in the heparanase gene (HPSE) have been shown to be associated with tumors. In this study, we investigated whether SNPs in HPSE were a risk factor for hepatocellular carcinoma (HCC) by undertaking a comprehensive haplotype-tagging, case-control study. For this, six haplotype-tagging SNPs (htSNPs) in HPSE were genotyped in 400 HCC patients and 480 controls by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. A log-additive model revealed significant correlations between the HPSE polymorphisms rs12331678 and rs12503843 and the risk of HCC in the overall samples (p = 0.0046 and p = 0.0055). When...